In vitro Testing

Dermaclaim has different in vitro models at your disposal:
- Human keratinocytes (HaCaT, NHEK)
- Normal Human Dermal Fibroblasts (NHDF)
- Reconstructed Human Epidermis (RHE, RHE FT)
- Normal Human Epidermal Melanocytes (NHEM)
- Reconstructed Human Pigmented Epidermis (RHPE)
- iPSC-derivates sebocytes
- Human Adipocytes (HPAd, HAd)
- Human Skeletal Muscle Cells (HSkMC)
- Human Follicle Dermal Papilla Cells (HFDPC)
- Human Hair Follicle Keratinocytes (HHFK)

In tubo colorimetric platforms or antiaging and antioxidant activities, we can evaluate the following biomarkers:
- Extracellular matrix proteins (collagen, elastin, HA, MMPs, versican…)
- Oxidative stress and DNA damage (ROS, MTT, thymine dimers, Y-H2AX)
- Senescence markers (telomeres, TERT, TEP1, B-gal, SIRT1)
- Oxidative damages (protein carbonylation, lipid peroxidation)
- mRNA gene expression (SOD1, CAT, GPx1, NRF2…)
- Glutathione levels (GSH-GSSG)
- Reactive Oxygen/Nytrogen Species (ROS and RNS)

For slimming and lipogenesis pathways, we can evaluate the following biomarkers:
- Total fatty acid content (Oil Red O staining)
- Lipogenesis mRNA gene expression (ATGL, HSL, PRDM, UCP…)
- Sebocyte differentiation and maturation (EMA, KRT7…)
- 5-alpha Reductase (SRD5A1-3) activity and testosterone metabolism
- Inflammatory response after induction

For skin pigmentation, we can evaluate the following biomarkers:
- Melanin content
- Melanogenesis pathways (TYR, TYRP-1, TYRP-2, MITF, POMC, Pmel17…)
- Tyrosinase enzymatic activity (in tubo)
- Fontana-Masson staining and image analysis

For cellular proliferation, barrier function, and healing, we can evaluate the following biomarkers:
- Cell cytotoxicity and proliferation (MTT, BrdU, NRU)
- Epidermal differentiation biomarkers (filaggrin, involucrin, cytokeratins…)
- Barrier function biomarkers (hyaluronic acid, ceramides, aquaporines, GAGs…)
- Wound-healing (Scratch-assay)
- Epidermal cohesion biomarkers (integrin, occludin, claudin, collagens, connexins…)

For hair efficacy, we can evaluate the following biomarkers:
- Cell proliferation and growth (MTT)
- 5alpha-Reductase (SRD5A1, SRD5A2, SRD5A3)
- Growth factors (EDN1, HDC, FGF1, VEGF, KGF, IGF1…)
- Specific marker expression (Ki67, B-catenin, apoptosis, KRTs…)
- Philpott Test (growth)
- Hair graying biomarkers (IRF4, MITF, TYR, TYRP-1)
- Protein degradation and oxidative stress protection

For inflammatory pathways, we can evaluate the following biomarkers, using or not external aggressors (LPS, histamine, UV, PMA):
- Inflammation biomarkers (TNFa, IL1a, IL1b, IL6, IL8, IL10, ICAM, TGFb…)
- Transcription factors (NFkB, AP1, STAT1, COX2, ALOX5…)
- Pain inhibition and signalling (TRPV1, TNFa)